BLASTN 2.3.0+
Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb
Miller (2000), "A greedy algorithm for aligning DNA sequences", J
Comput Biol 2000; 7(1-2):203-14.
Database: homo-hairpin.fasta
1,881 sequences; 154,002 total letters
Query= TTCCTGCCTGGTCGGCCTGAAGGGAAGGGCCAAT
Length=34
Score E
Sequences producing significant alignments: (Bits) Value
[3415]hsa-mir-628 MI0003642 Homo sapiens miR-628 stem-loop 23.3 0.25
[5117]hsa-mir-1178 MI0006271 Homo sapiens miR-1178 stem-loop 21.4 0.90
[2949]hsa-mir-518e MI0003169 Homo sapiens miR-518e stem-loop 21.4 0.90
>[3415]hsa-mir-628 MI0003642 Homo sapiens miR-628 stem-loop
Length=95
Score = 23.3 bits (12), Expect = 0.25
Identities = 12/12 (100%), Gaps = 0/12 (0%)
Strand=Plus/Plus
Query 19 GAAGGGAAGGGC 30
||||||||||||
Sbjct 80 GAAGGGAAGGGC 91
>[5117]hsa-mir-1178 MI0006271 Homo sapiens miR-1178 stem-loop
Length=91
Score = 21.4 bits (11), Expect = 0.90
Identities = 14/15 (93%), Gaps = 1/15 (7%)
Strand=Plus/Plus
Query 19 GAAGGGAAGGG-CCA 32
||||||||||| |||
Sbjct 17 GAAGGGAAGGGTCCA 31
>[2949]hsa-mir-518e MI0003169 Homo sapiens miR-518e stem-loop
Length=88
Score = 21.4 bits (11), Expect = 0.90
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 17 CTGAAGGGAAG 27
|||||||||||
Sbjct 70 CTGAAGGGAAG 60
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 2553360
Database: homo-hairpin.fasta
Posted date: Sep 23, 2016 6:16 PM
Number of letters in database: 154,002
Number of sequences in database: 1,881
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5