BLASTN 2.3.0+
Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb
Miller (2000), "A greedy algorithm for aligning DNA sequences", J
Comput Biol 2000; 7(1-2):203-14.
Database: homo-hairpin.fasta
1,881 sequences; 154,002 total letters
Query= TCCCCGGCCGAACCTGCCCGACCCTCCCTCCCCC
Length=34
Score E
Sequences producing significant alignments: (Bits) Value
[3435]hsa-mir-647 MI0003662 Homo sapiens miR-647 stem-loop 30.7 0.001
[447]hsa-mir-149 MI0000478 Homo sapiens miR-149 stem-loop 25.1 0.070
[20811]hsa-mir-6775 MI0022620 Homo sapiens miR-6775 stem-loop 23.3 0.25
[23671]hsa-mir-7845 MI0025515 Homo sapiens miR-7845 stem-loop 21.4 0.90
[15843]hsa-mir-4769 MI0017410 Homo sapiens miR-4769 stem-loop 21.4 0.90
>[3435]hsa-mir-647 MI0003662 Homo sapiens miR-647 stem-loop
Length=96
Score = 30.7 bits (16), Expect = 0.001
Identities = 18/19 (95%), Gaps = 0/19 (0%)
Strand=Plus/Minus
Query 13 CCTGCCCGACCCTCCCTCC 31
|||||| ||||||||||||
Sbjct 82 CCTGCCTGACCCTCCCTCC 64
>[447]hsa-mir-149 MI0000478 Homo sapiens miR-149 stem-loop
Length=89
Score = 25.1 bits (13), Expect = 0.070
Identities = 15/16 (94%), Gaps = 0/16 (0%)
Strand=Plus/Minus
Query 17 CCCGACCCTCCCTCCC 32
|||| |||||||||||
Sbjct 68 CCCGTCCCTCCCTCCC 53
>[20811]hsa-mir-6775 MI0022620 Homo sapiens miR-6775 stem-loop
Length=69
Score = 23.3 bits (12), Expect = 0.25
Identities = 19/22 (86%), Gaps = 1/22 (5%)
Strand=Plus/Minus
Query 13 CCTGCCCGACCCTCCCTCCCCC 34
|||| || | ||||||||||||
Sbjct 35 CCTGTCC-AGCCTCCCTCCCCC 15
>[23671]hsa-mir-7845 MI0025515 Homo sapiens miR-7845 stem-loop
Length=99
Score = 21.4 bits (11), Expect = 0.90
Identities = 14/15 (93%), Gaps = 1/15 (7%)
Strand=Plus/Minus
Query 16 GCC-CGACCCTCCCT 29
||| |||||||||||
Sbjct 24 GCCACGACCCTCCCT 10
>[15843]hsa-mir-4769 MI0017410 Homo sapiens miR-4769 stem-loop
Length=77
Score = 21.4 bits (11), Expect = 0.90
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 23 CCTCCCTCCCC 33
|||||||||||
Sbjct 56 CCTCCCTCCCC 66
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 2553360
Database: homo-hairpin.fasta
Posted date: Sep 23, 2016 6:16 PM
Number of letters in database: 154,002
Number of sequences in database: 1,881
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5