BLASTN 2.3.0+
Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb
Miller (2000), "A greedy algorithm for aligning DNA sequences", J
Comput Biol 2000; 7(1-2):203-14.
Database: homo-hairpin.fasta
1,881 sequences; 154,002 total letters
Query= GAACCTTAGCGGACCCTGGGAGGAGGCTCCCCGGCCGAACCTGCCCGACCCTCCCTC
Length=57
Score E
Sequences producing significant alignments: (Bits) Value
[3435]hsa-mir-647 MI0003662 Homo sapiens miR-647 stem-loop 28.8 0.011
[20789]hsa-mir-6753 MI0022598 Homo sapiens miR-6753 stem-loop 23.3 0.52
[17514]hsa-mir-3680-2 MI0019113 Homo sapiens miR-3680-2 stem-loop 23.3 0.52
[14522]hsa-mir-3680-1 MI0016081 Homo sapiens miR-3680-1 stem-loop 23.3 0.52
[23671]hsa-mir-7845 MI0025515 Homo sapiens miR-7845 stem-loop 21.4 1.9
[21153]hsa-mir-7111 MI0022962 Homo sapiens miR-7111 stem-loop 21.4 1.9
[21148]hsa-mir-7106 MI0022957 Homo sapiens miR-7106 stem-loop 21.4 1.9
[20800]hsa-mir-6764 MI0022609 Homo sapiens miR-6764 stem-loop 21.4 1.9
[20799]hsa-mir-6763 MI0022608 Homo sapiens miR-6763 stem-loop 21.4 1.9
[4446]hsa-mir-877 MI0005561 Homo sapiens miR-877 stem-loop 21.4 1.9
>[3435]hsa-mir-647 MI0003662 Homo sapiens miR-647 stem-loop
Length=96
Score = 28.8 bits (15), Expect = 0.011
Identities = 17/18 (94%), Gaps = 0/18 (0%)
Strand=Plus/Minus
Query 40 CCTGCCCGACCCTCCCTC 57
|||||| |||||||||||
Sbjct 82 CCTGCCTGACCCTCCCTC 65
>[20789]hsa-mir-6753 MI0022598 Homo sapiens miR-6753 stem-loop
Length=164
Score = 23.3 bits (12), Expect = 0.52
Identities = 17/19 (89%), Gaps = 2/19 (11%)
Strand=Plus/Minus
Query 4 CCTT-AGCGGACCCTGGGA 21
|||| || |||||||||||
Sbjct 123 CCTTCAG-GGACCCTGGGA 106
>[17514]hsa-mir-3680-2 MI0019113 Homo sapiens miR-3680-2 stem-loop
Length=87
Score = 23.3 bits (12), Expect = 0.52
Identities = 14/15 (93%), Gaps = 0/15 (0%)
Strand=Plus/Plus
Query 12 GACCCTGGGAGGAGG 26
||||||||||| |||
Sbjct 58 GACCCTGGGAGTAGG 72
>[14522]hsa-mir-3680-1 MI0016081 Homo sapiens miR-3680-1 stem-loop
Length=87
Score = 23.3 bits (12), Expect = 0.52
Identities = 14/15 (93%), Gaps = 0/15 (0%)
Strand=Plus/Plus
Query 12 GACCCTGGGAGGAGG 26
||||||||||| |||
Sbjct 58 GACCCTGGGAGTAGG 72
>[23671]hsa-mir-7845 MI0025515 Homo sapiens miR-7845 stem-loop
Length=99
Score = 21.4 bits (11), Expect = 1.9
Identities = 14/15 (93%), Gaps = 1/15 (7%)
Strand=Plus/Minus
Query 43 GCC-CGACCCTCCCT 56
||| |||||||||||
Sbjct 24 GCCACGACCCTCCCT 10
>[21153]hsa-mir-7111 MI0022962 Homo sapiens miR-7111 stem-loop
Length=72
Score = 21.4 bits (11), Expect = 1.9
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 16 CTGGGAGGAGG 26
|||||||||||
Sbjct 72 CTGGGAGGAGG 62
>[21148]hsa-mir-7106 MI0022957 Homo sapiens miR-7106 stem-loop
Length=65
Score = 21.4 bits (11), Expect = 1.9
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 16 CTGGGAGGAGG 26
|||||||||||
Sbjct 5 CTGGGAGGAGG 15
>[20800]hsa-mir-6764 MI0022609 Homo sapiens miR-6764 stem-loop
Length=61
Score = 21.4 bits (11), Expect = 1.9
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 12 GACCCTGGGAG 22
|||||||||||
Sbjct 15 GACCCTGGGAG 5
>[20799]hsa-mir-6763 MI0022608 Homo sapiens miR-6763 stem-loop
Length=65
Score = 21.4 bits (11), Expect = 1.9
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 26 GCTCCCCGGCC 36
|||||||||||
Sbjct 44 GCTCCCCGGCC 54
>[4446]hsa-mir-877 MI0005561 Homo sapiens miR-877 stem-loop
Length=86
Score = 21.4 bits (11), Expect = 1.9
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 16 CTGGGAGGAGG 26
|||||||||||
Sbjct 86 CTGGGAGGAGG 76
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 5283054
Database: homo-hairpin.fasta
Posted date: Sep 23, 2016 6:16 PM
Number of letters in database: 154,002
Number of sequences in database: 1,881
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5