BLASTN 2.3.0+
Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb
Miller (2000), "A greedy algorithm for aligning DNA sequences", J
Comput Biol 2000; 7(1-2):203-14.
Database: homo-hairpin.fasta
1,881 sequences; 154,002 total letters
Query= AGCAGAGGCTGAAGAGGGTGGGGAAGCAGGGGAGCTGTGCGTGTGTCGGGGCGGGTGGAACCTTAGCG
GACCCTGGGAGGAGGCTCCCCGGCCGAACCTGCCCGACCCTCCCTCCC
Length=116
Score E
Sequences producing significant alignments: (Bits) Value
[3435]hsa-mir-647 MI0003662 Homo sapiens miR-647 stem-loop 30.7 0.007
[20789]hsa-mir-6753 MI0022598 Homo sapiens miR-6753 stem-loop 25.1 0.34
[4579]hsa-mir-920 MI0005712 Homo sapiens miR-920 stem-loop 25.1 0.34
[447]hsa-mir-149 MI0000478 Homo sapiens miR-149 stem-loop 25.1 0.34
[20922]hsa-mir-6884 MI0022731 Homo sapiens miR-6884 stem-loop 23.3 1.2
[17514]hsa-mir-3680-2 MI0019113 Homo sapiens miR-3680-2 stem-loop 23.3 1.2
[15329]hsa-mir-4525 MI0016892 Homo sapiens miR-4525 stem-loop 23.3 1.2
[14522]hsa-mir-3680-1 MI0016081 Homo sapiens miR-3680-1 stem-loop 23.3 1.2
[23671]hsa-mir-7845 MI0025515 Homo sapiens miR-7845 stem-loop 21.4 4.4
[21153]hsa-mir-7111 MI0022962 Homo sapiens miR-7111 stem-loop 21.4 4.4
[21148]hsa-mir-7106 MI0022957 Homo sapiens miR-7106 stem-loop 21.4 4.4
[20915]hsa-mir-6877 MI0022724 Homo sapiens miR-6877 stem-loop 21.4 4.4
[20822]hsa-mir-6786 MI0022631 Homo sapiens miR-6786 stem-loop 21.4 4.4
[20800]hsa-mir-6764 MI0022609 Homo sapiens miR-6764 stem-loop 21.4 4.4
[20799]hsa-mir-6763 MI0022608 Homo sapiens miR-6763 stem-loop 21.4 4.4
[16298]hsa-mir-5004 MI0017870 Homo sapiens miR-5004 stem-loop 21.4 4.4
[14867]hsa-mir-3150b MI0016426 Homo sapiens miR-3150b stem-loop 21.4 4.4
[14533]hsa-mir-3691 MI0016092 Homo sapiens miR-3691 stem-loop 21.4 4.4
[14497]hsa-mir-3653 MI0016053 Homo sapiens miR-3653 stem-loop 21.4 4.4
[12663]hsa-mir-3141 MI0014165 Homo sapiens miR-3141 stem-loop 21.4 4.4
[4446]hsa-mir-877 MI0005561 Homo sapiens miR-877 stem-loop 21.4 4.4
[732]hsa-mir-370 MI0000778 Homo sapiens miR-370 stem-loop 21.4 4.4
[268]hsa-mir-210 MI0000286 Homo sapiens miR-210 stem-loop 21.4 4.4
>[3435]hsa-mir-647 MI0003662 Homo sapiens miR-647 stem-loop
Length=96
Score = 30.7 bits (16), Expect = 0.007
Identities = 18/19 (95%), Gaps = 0/19 (0%)
Strand=Plus/Minus
Query 97 CCTGCCCGACCCTCCCTCC 115
|||||| ||||||||||||
Sbjct 82 CCTGCCTGACCCTCCCTCC 64
>[20789]hsa-mir-6753 MI0022598 Homo sapiens miR-6753 stem-loop
Length=164
Score = 25.1 bits (13), Expect = 0.34
Identities = 28/34 (82%), Gaps = 6/34 (18%)
Strand=Plus/Minus
Query 47 CGG-GGCGGGTGGAACCTT-AGCGGACCCTGGGA 78
||| |||||| || |||| || |||||||||||
Sbjct 135 CGGTGGCGGG-GG--CCTTCAG-GGACCCTGGGA 106
>[4579]hsa-mir-920 MI0005712 Homo sapiens miR-920 stem-loop
Length=75
Score = 25.1 bits (13), Expect = 0.34
Identities = 13/13 (100%), Gaps = 0/13 (0%)
Strand=Plus/Plus
Query 27 CAGGGGAGCTGTG 39
|||||||||||||
Sbjct 49 CAGGGGAGCTGTG 61
>[447]hsa-mir-149 MI0000478 Homo sapiens miR-149 stem-loop
Length=89
Score = 25.1 bits (13), Expect = 0.34
Identities = 15/16 (94%), Gaps = 0/16 (0%)
Strand=Plus/Minus
Query 101 CCCGACCCTCCCTCCC 116
|||| |||||||||||
Sbjct 68 CCCGTCCCTCCCTCCC 53
>[20922]hsa-mir-6884 MI0022731 Homo sapiens miR-6884 stem-loop
Length=78
Score = 23.3 bits (12), Expect = 1.2
Identities = 17/19 (89%), Gaps = 1/19 (5%)
Strand=Plus/Plus
Query 2 GCAGAGGCTGAAGAGGGTG 20
||||||||||| || ||||
Sbjct 4 GCAGAGGCTGA-GAAGGTG 21
>[17514]hsa-mir-3680-2 MI0019113 Homo sapiens miR-3680-2 stem-loop
Length=87
Score = 23.3 bits (12), Expect = 1.2
Identities = 14/15 (93%), Gaps = 0/15 (0%)
Strand=Plus/Plus
Query 69 GACCCTGGGAGGAGG 83
||||||||||| |||
Sbjct 58 GACCCTGGGAGTAGG 72
>[15329]hsa-mir-4525 MI0016892 Homo sapiens miR-4525 stem-loop
Length=75
Score = 23.3 bits (12), Expect = 1.2
Identities = 12/12 (100%), Gaps = 0/12 (0%)
Strand=Plus/Minus
Query 15 AGGGTGGGGAAG 26
||||||||||||
Sbjct 72 AGGGTGGGGAAG 61
>[14522]hsa-mir-3680-1 MI0016081 Homo sapiens miR-3680-1 stem-loop
Length=87
Score = 23.3 bits (12), Expect = 1.2
Identities = 14/15 (93%), Gaps = 0/15 (0%)
Strand=Plus/Plus
Query 69 GACCCTGGGAGGAGG 83
||||||||||| |||
Sbjct 58 GACCCTGGGAGTAGG 72
>[23671]hsa-mir-7845 MI0025515 Homo sapiens miR-7845 stem-loop
Length=99
Score = 21.4 bits (11), Expect = 4.4
Identities = 14/15 (93%), Gaps = 1/15 (7%)
Strand=Plus/Minus
Query 100 GCC-CGACCCTCCCT 113
||| |||||||||||
Sbjct 24 GCCACGACCCTCCCT 10
>[21153]hsa-mir-7111 MI0022962 Homo sapiens miR-7111 stem-loop
Length=72
Score = 21.4 bits (11), Expect = 4.4
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 73 CTGGGAGGAGG 83
|||||||||||
Sbjct 72 CTGGGAGGAGG 62
>[21148]hsa-mir-7106 MI0022957 Homo sapiens miR-7106 stem-loop
Length=65
Score = 21.4 bits (11), Expect = 4.4
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 73 CTGGGAGGAGG 83
|||||||||||
Sbjct 5 CTGGGAGGAGG 15
>[20915]hsa-mir-6877 MI0022724 Homo sapiens miR-6877 stem-loop
Length=64
Score = 21.4 bits (11), Expect = 4.4
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 2 GCAGAGGCTGA 12
|||||||||||
Sbjct 49 GCAGAGGCTGA 39
>[20822]hsa-mir-6786 MI0022631 Homo sapiens miR-6786 stem-loop
Length=113
Score = 21.4 bits (11), Expect = 4.4
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 27 CAGGGGAGCTG 37
|||||||||||
Sbjct 44 CAGGGGAGCTG 34
>[20800]hsa-mir-6764 MI0022609 Homo sapiens miR-6764 stem-loop
Length=61
Score = 21.4 bits (11), Expect = 4.4
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 69 GACCCTGGGAG 79
|||||||||||
Sbjct 15 GACCCTGGGAG 5
>[20799]hsa-mir-6763 MI0022608 Homo sapiens miR-6763 stem-loop
Length=65
Score = 21.4 bits (11), Expect = 4.4
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 83 GCTCCCCGGCC 93
|||||||||||
Sbjct 44 GCTCCCCGGCC 54
>[16298]hsa-mir-5004 MI0017870 Homo sapiens miR-5004 stem-loop
Length=107
Score = 21.4 bits (11), Expect = 4.4
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 2 GCAGAGGCTGA 12
|||||||||||
Sbjct 54 GCAGAGGCTGA 64
>[14867]hsa-mir-3150b MI0016426 Homo sapiens miR-3150b stem-loop
Length=86
Score = 21.4 bits (11), Expect = 4.4
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 20 GGGGAAGCAGG 30
|||||||||||
Sbjct 84 GGGGAAGCAGG 74
>[14533]hsa-mir-3691 MI0016092 Homo sapiens miR-3691 stem-loop
Length=90
Score = 21.4 bits (11), Expect = 4.4
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 14 GAGGGTGGGGA 24
|||||||||||
Sbjct 46 GAGGGTGGGGA 56
>[14497]hsa-mir-3653 MI0016053 Homo sapiens miR-3653 stem-loop
Length=110
Score = 21.4 bits (11), Expect = 4.4
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 19 TGGGGAAGCAG 29
|||||||||||
Sbjct 106 TGGGGAAGCAG 96
>[12663]hsa-mir-3141 MI0014165 Homo sapiens miR-3141 stem-loop
Length=61
Score = 21.4 bits (11), Expect = 4.4
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 49 GGGCGGGTGGA 59
|||||||||||
Sbjct 12 GGGCGGGTGGA 22
>[4446]hsa-mir-877 MI0005561 Homo sapiens miR-877 stem-loop
Length=86
Score = 21.4 bits (11), Expect = 4.4
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 73 CTGGGAGGAGG 83
|||||||||||
Sbjct 86 CTGGGAGGAGG 76
>[732]hsa-mir-370 MI0000778 Homo sapiens miR-370 stem-loop
Length=75
Score = 21.4 bits (11), Expect = 4.4
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 53 GGGTGGAACCT 63
|||||||||||
Sbjct 56 GGGTGGAACCT 66
>[268]hsa-mir-210 MI0000286 Homo sapiens miR-210 stem-loop
Length=110
Score = 21.4 bits (11), Expect = 4.4
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 35 CTGTGCGTGTG 45
|||||||||||
Sbjct 66 CTGTGCGTGTG 76
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 12390600
Database: homo-hairpin.fasta
Posted date: Sep 23, 2016 6:16 PM
Number of letters in database: 154,002
Number of sequences in database: 1,881
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5