BLASTN 2.3.0+
Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb
Miller (2000), "A greedy algorithm for aligning DNA sequences", J
Comput Biol 2000; 7(1-2):203-14.
Database: homo-hairpin.fasta
1,881 sequences; 154,002 total letters
Query= AGCAGAGGCTGAAGAGGGTGGGGAAGCAGGGGAGCTGTGCGTGTGTCGGGGCGGGTGGAACCTTAGCG
GACCCTGGGAGG
Length=80
Score E
Sequences producing significant alignments: (Bits) Value
[20789]hsa-mir-6753 MI0022598 Homo sapiens miR-6753 stem-loop 25.1 0.22
[4579]hsa-mir-920 MI0005712 Homo sapiens miR-920 stem-loop 25.1 0.22
[20922]hsa-mir-6884 MI0022731 Homo sapiens miR-6884 stem-loop 23.3 0.80
[15329]hsa-mir-4525 MI0016892 Homo sapiens miR-4525 stem-loop 23.3 0.80
[20915]hsa-mir-6877 MI0022724 Homo sapiens miR-6877 stem-loop 21.4 2.9
[20822]hsa-mir-6786 MI0022631 Homo sapiens miR-6786 stem-loop 21.4 2.9
[20800]hsa-mir-6764 MI0022609 Homo sapiens miR-6764 stem-loop 21.4 2.9
[17514]hsa-mir-3680-2 MI0019113 Homo sapiens miR-3680-2 stem-loop 21.4 2.9
[16298]hsa-mir-5004 MI0017870 Homo sapiens miR-5004 stem-loop 21.4 2.9
[14867]hsa-mir-3150b MI0016426 Homo sapiens miR-3150b stem-loop 21.4 2.9
[14533]hsa-mir-3691 MI0016092 Homo sapiens miR-3691 stem-loop 21.4 2.9
[14522]hsa-mir-3680-1 MI0016081 Homo sapiens miR-3680-1 stem-loop 21.4 2.9
[14497]hsa-mir-3653 MI0016053 Homo sapiens miR-3653 stem-loop 21.4 2.9
[12663]hsa-mir-3141 MI0014165 Homo sapiens miR-3141 stem-loop 21.4 2.9
[732]hsa-mir-370 MI0000778 Homo sapiens miR-370 stem-loop 21.4 2.9
[268]hsa-mir-210 MI0000286 Homo sapiens miR-210 stem-loop 21.4 2.9
>[20789]hsa-mir-6753 MI0022598 Homo sapiens miR-6753 stem-loop
Length=164
Score = 25.1 bits (13), Expect = 0.22
Identities = 28/34 (82%), Gaps = 6/34 (18%)
Strand=Plus/Minus
Query 47 CGG-GGCGGGTGGAACCTT-AGCGGACCCTGGGA 78
||| |||||| || |||| || |||||||||||
Sbjct 135 CGGTGGCGGG-GG--CCTTCAG-GGACCCTGGGA 106
>[4579]hsa-mir-920 MI0005712 Homo sapiens miR-920 stem-loop
Length=75
Score = 25.1 bits (13), Expect = 0.22
Identities = 13/13 (100%), Gaps = 0/13 (0%)
Strand=Plus/Plus
Query 27 CAGGGGAGCTGTG 39
|||||||||||||
Sbjct 49 CAGGGGAGCTGTG 61
>[20922]hsa-mir-6884 MI0022731 Homo sapiens miR-6884 stem-loop
Length=78
Score = 23.3 bits (12), Expect = 0.80
Identities = 17/19 (89%), Gaps = 1/19 (5%)
Strand=Plus/Plus
Query 2 GCAGAGGCTGAAGAGGGTG 20
||||||||||| || ||||
Sbjct 4 GCAGAGGCTGA-GAAGGTG 21
>[15329]hsa-mir-4525 MI0016892 Homo sapiens miR-4525 stem-loop
Length=75
Score = 23.3 bits (12), Expect = 0.80
Identities = 12/12 (100%), Gaps = 0/12 (0%)
Strand=Plus/Minus
Query 15 AGGGTGGGGAAG 26
||||||||||||
Sbjct 72 AGGGTGGGGAAG 61
>[20915]hsa-mir-6877 MI0022724 Homo sapiens miR-6877 stem-loop
Length=64
Score = 21.4 bits (11), Expect = 2.9
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 2 GCAGAGGCTGA 12
|||||||||||
Sbjct 49 GCAGAGGCTGA 39
>[20822]hsa-mir-6786 MI0022631 Homo sapiens miR-6786 stem-loop
Length=113
Score = 21.4 bits (11), Expect = 2.9
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 27 CAGGGGAGCTG 37
|||||||||||
Sbjct 44 CAGGGGAGCTG 34
>[20800]hsa-mir-6764 MI0022609 Homo sapiens miR-6764 stem-loop
Length=61
Score = 21.4 bits (11), Expect = 2.9
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 69 GACCCTGGGAG 79
|||||||||||
Sbjct 15 GACCCTGGGAG 5
>[17514]hsa-mir-3680-2 MI0019113 Homo sapiens miR-3680-2 stem-loop
Length=87
Score = 21.4 bits (11), Expect = 2.9
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 69 GACCCTGGGAG 79
|||||||||||
Sbjct 58 GACCCTGGGAG 68
>[16298]hsa-mir-5004 MI0017870 Homo sapiens miR-5004 stem-loop
Length=107
Score = 21.4 bits (11), Expect = 2.9
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 2 GCAGAGGCTGA 12
|||||||||||
Sbjct 54 GCAGAGGCTGA 64
>[14867]hsa-mir-3150b MI0016426 Homo sapiens miR-3150b stem-loop
Length=86
Score = 21.4 bits (11), Expect = 2.9
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 20 GGGGAAGCAGG 30
|||||||||||
Sbjct 84 GGGGAAGCAGG 74
>[14533]hsa-mir-3691 MI0016092 Homo sapiens miR-3691 stem-loop
Length=90
Score = 21.4 bits (11), Expect = 2.9
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 14 GAGGGTGGGGA 24
|||||||||||
Sbjct 46 GAGGGTGGGGA 56
>[14522]hsa-mir-3680-1 MI0016081 Homo sapiens miR-3680-1 stem-loop
Length=87
Score = 21.4 bits (11), Expect = 2.9
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 69 GACCCTGGGAG 79
|||||||||||
Sbjct 58 GACCCTGGGAG 68
>[14497]hsa-mir-3653 MI0016053 Homo sapiens miR-3653 stem-loop
Length=110
Score = 21.4 bits (11), Expect = 2.9
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 19 TGGGGAAGCAG 29
|||||||||||
Sbjct 106 TGGGGAAGCAG 96
>[12663]hsa-mir-3141 MI0014165 Homo sapiens miR-3141 stem-loop
Length=61
Score = 21.4 bits (11), Expect = 2.9
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 49 GGGCGGGTGGA 59
|||||||||||
Sbjct 12 GGGCGGGTGGA 22
>[732]hsa-mir-370 MI0000778 Homo sapiens miR-370 stem-loop
Length=75
Score = 21.4 bits (11), Expect = 2.9
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 53 GGGTGGAACCT 63
|||||||||||
Sbjct 56 GGGTGGAACCT 66
>[268]hsa-mir-210 MI0000286 Homo sapiens miR-210 stem-loop
Length=110
Score = 21.4 bits (11), Expect = 2.9
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 35 CTGTGCGTGTG 45
|||||||||||
Sbjct 66 CTGTGCGTGTG 76
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 8176155
Database: homo-hairpin.fasta
Posted date: Sep 23, 2016 6:16 PM
Number of letters in database: 154,002
Number of sequences in database: 1,881
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5