BLASTN 2.3.0+
Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb
Miller (2000), "A greedy algorithm for aligning DNA sequences", J
Comput Biol 2000; 7(1-2):203-14.
Database: homo-hairpin.fasta
1,881 sequences; 154,002 total letters
Query= TGGTTTACAGAACCCTTTTAAGAACTGTAAGCCTTGTGAGGTTCGGCAGGTGTTATTTTCCTCTTTGC
AGTTGGGAAACTGAAG
Length=84
Score E
Sequences producing significant alignments: (Bits) Value
[15243]hsa-mir-4460 MI0016806 Homo sapiens miR-4460 stem-loop 25.1 0.24
[761]hsa-mir-323a MI0000807 Homo sapiens miR-323a stem-loop 23.3 0.85
[15295]hsa-mir-4496 MI0016858 Homo sapiens miR-4496 stem-loop 21.4 3.1
[5257]hsa-mir-302e MI0006417 Homo sapiens miR-302e stem-loop 21.4 3.1
>[15243]hsa-mir-4460 MI0016806 Homo sapiens miR-4460 stem-loop
Length=86
Score = 25.1 bits (13), Expect = 0.24
Identities = 13/13 (100%), Gaps = 0/13 (0%)
Strand=Plus/Plus
Query 59 TCCTCTTTGCAGT 71
|||||||||||||
Sbjct 35 TCCTCTTTGCAGT 47
>[761]hsa-mir-323a MI0000807 Homo sapiens miR-323a stem-loop
Length=86
Score = 23.3 bits (12), Expect = 0.85
Identities = 12/12 (100%), Gaps = 0/12 (0%)
Strand=Plus/Plus
Query 60 CCTCTTTGCAGT 71
||||||||||||
Sbjct 67 CCTCTTTGCAGT 78
>[15295]hsa-mir-4496 MI0016858 Homo sapiens miR-4496 stem-loop
Length=61
Score = 21.4 bits (11), Expect = 3.1
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Plus
Query 74 GGAAACTGAAG 84
|||||||||||
Sbjct 42 GGAAACTGAAG 52
>[5257]hsa-mir-302e MI0006417 Homo sapiens miR-302e stem-loop
Length=72
Score = 21.4 bits (11), Expect = 3.1
Identities = 22/27 (81%), Gaps = 1/27 (4%)
Strand=Plus/Minus
Query 59 TCC-TCTTTGCAGTTGGGAAACTGAAG 84
||| |||| |||| |||||||||||
Sbjct 46 TCCATCTTACCAGTAAGGAAACTGAAG 20
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 8679303
Database: homo-hairpin.fasta
Posted date: Sep 23, 2016 6:16 PM
Number of letters in database: 154,002
Number of sequences in database: 1,881
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5