BLASTN 2.3.0+
Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb
Miller (2000), "A greedy algorithm for aligning DNA sequences", J
Comput Biol 2000; 7(1-2):203-14.
Database: homo-hairpin.fasta
1,881 sequences; 154,002 total letters
Query= ATGGTTTACAGAACCCTTTTAAGAACTGTAAGCCTTGTGAGGTTCGGCAGGTGTTATTTTCCTCTTTG
CAGTTGGGAAACTGAAGC
Length=86
Score E
Sequences producing significant alignments: (Bits) Value
[15243]hsa-mir-4460 MI0016806 Homo sapiens miR-4460 stem-loop 25.1 0.24
[15295]hsa-mir-4496 MI0016858 Homo sapiens miR-4496 stem-loop 23.3 0.88
[5257]hsa-mir-302e MI0006417 Homo sapiens miR-302e stem-loop 23.3 0.88
[761]hsa-mir-323a MI0000807 Homo sapiens miR-323a stem-loop 23.3 0.88
[15846]hsa-mir-4771-2 MI0017413 Homo sapiens miR-4771-2 stem-loop 21.4 3.2
[15845]hsa-mir-4771-1 MI0017412 Homo sapiens miR-4771-1 stem-loop 21.4 3.2
>[15243]hsa-mir-4460 MI0016806 Homo sapiens miR-4460 stem-loop
Length=86
Score = 25.1 bits (13), Expect = 0.24
Identities = 13/13 (100%), Gaps = 0/13 (0%)
Strand=Plus/Plus
Query 60 TCCTCTTTGCAGT 72
|||||||||||||
Sbjct 35 TCCTCTTTGCAGT 47
>[15295]hsa-mir-4496 MI0016858 Homo sapiens miR-4496 stem-loop
Length=61
Score = 23.3 bits (12), Expect = 0.88
Identities = 12/12 (100%), Gaps = 0/12 (0%)
Strand=Plus/Plus
Query 75 GGAAACTGAAGC 86
||||||||||||
Sbjct 42 GGAAACTGAAGC 53
>[5257]hsa-mir-302e MI0006417 Homo sapiens miR-302e stem-loop
Length=72
Score = 23.3 bits (12), Expect = 0.88
Identities = 23/28 (82%), Gaps = 1/28 (4%)
Strand=Plus/Minus
Query 60 TCC-TCTTTGCAGTTGGGAAACTGAAGC 86
||| |||| |||| ||||||||||||
Sbjct 46 TCCATCTTACCAGTAAGGAAACTGAAGC 19
>[761]hsa-mir-323a MI0000807 Homo sapiens miR-323a stem-loop
Length=86
Score = 23.3 bits (12), Expect = 0.88
Identities = 12/12 (100%), Gaps = 0/12 (0%)
Strand=Plus/Plus
Query 61 CCTCTTTGCAGT 72
||||||||||||
Sbjct 67 CCTCTTTGCAGT 78
>[15846]hsa-mir-4771-2 MI0017413 Homo sapiens miR-4771-2 stem-loop
Length=74
Score = 21.4 bits (11), Expect = 3.2
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 76 GAAACTGAAGC 86
|||||||||||
Sbjct 38 GAAACTGAAGC 28
>[15845]hsa-mir-4771-1 MI0017412 Homo sapiens miR-4771-1 stem-loop
Length=74
Score = 21.4 bits (11), Expect = 3.2
Identities = 11/11 (100%), Gaps = 0/11 (0%)
Strand=Plus/Minus
Query 76 GAAACTGAAGC 86
|||||||||||
Sbjct 38 GAAACTGAAGC 28
Lambda K H
1.33 0.621 1.12
Gapped
Lambda K H
1.28 0.460 0.850
Effective search space used: 8930877
Database: homo-hairpin.fasta
Posted date: Sep 23, 2016 6:16 PM
Number of letters in database: 154,002
Number of sequences in database: 1,881
Matrix: blastn matrix 1 -2
Gap Penalties: Existence: 0, Extension: 2.5